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KMID : 0620920110430060367
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Experimental & Molecular Medicine
2011 Volume.43 No. 6 p.367 ~ p.373
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Effects of combined mechanical stimulation on the proliferation and differentiation of pre-osteoblasts
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Kang Kyung-Shin
Lee Seung-Jae
Lee Hak-Sue
Moon Won-Kyu
Cho Dong-Woo
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Abstract
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We observed how combined mechanical stimuli affect the proliferation and differentiation of pre-osteoblasts. For this research, a bioreactor system was developed that can simultaneously stimulate cells with cyclic strain and ultrasound, each of which is known to effectively stimulate bone tissue regeneration. MC3T3-E1 pre-osteoblasts were chosen for bone tissue engineering due to their osteoblast-like characteristics. 3-D scaffolds were fabricated with polycaprolactone (PCL) and poly-L-lactic acid (PLLA) using the salt leaching method. The cells were stimulated by the bioreactor with cyclic strain and ultrasound. The bioreactor was set at a frequency of 1.0 Hz and 10 % strain for cyclic strain and 1.0 MHz and 30 mW/cm2 for ultrasound. Three experimental groups (ultrasound, cyclic strain, and combined stimulation) and a control group were examined. Each group was stimulated for 20 min/day. Mechanical stimuli did not affect MC3T3-E1 cell proliferation significantly up to 10 days when measured with the cell counting kit-8. However, gene expression analysis of collagen type-I (COL-I), osteocalcin (OC), RUNX2, and osterix (OSX) revealed that the combined mechanical stimulation accelerated the matrix maturation of MC3T3-E1 cells. These results indicate that the combined mechanical stimulation can enhance the differentiation of pre-osteoblasts more efficiently than simple stimuli, in spite of no effect on cell proliferation.
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KEYWORD
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bioreactors, cell differentiation, cyclic strain, physical stimulation, tissue engineering, ultrasound
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